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验证数据展示

  • WB analysis of K-562 using 16031-1-AP (same clone as 16031-1-PBS)

    WB analysis of K-562 using 16031-1-AP (same clone as 16031-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16031-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 16031-1-AP (same clone as 16031-1-PBS)

    IP experiment of K-562 using 16031-1-AP (same clone as 16031-1-PBS)

    IP result of anti-RNF2 (IP:16031-1-AP, 4ug; Detection:16031-1-AP 1:800) with K-562 cells lysate 1200ug. This data was developed using the same antibody clone with 16031-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 16031-1-AP (same clone as 16031-1-PBS)

    IF Staining of HeLa using 16031-1-AP (same clone as 16031-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16031-1-AP (RNF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16031-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 16031-1-AP (same clone as 16031-1-PBS)

    WB analysis of HEK-293 using 16031-1-AP (same clone as 16031-1-PBS)

    WB result of RNF2 antibody (16031-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF2 transfected HEK-293 cells. This data was developed using the same antibody clone with 16031-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RNF2 Polyclonal antibody, PBS Only

RNF2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 16031-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

HIP2 interacting protein 3, EC:2.3.2.27, E3 ubiquitin-protein ligase RING2, DING, BAP1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RNF2 Ready-To-Use IHC Kit. RNF2 primary antibody included.

产品信息

16031-1-PBS targets RNF2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8900

Product name: Recombinant human RNF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-336 aa of BC012583

Sequence: MSQAVQTNGTQPLSKTWELSLYELQRTPQEAITDGLEIVVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCADCIITALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSRDEYEAHQERVLARINKHNNQQALSHSIEEGLKIQAMNRLQRGKKQQIENGSGAEDNGDSSHCSNASTHSNQEAGPSNKRTKTSDDSGLELDNNNAAMAIDPVMDGASEIELVFRPHPTLMEKDDSAQTRYIKTSGNATVDHLSKYLAVRLALEELRSKGESNQMNLDTASEKQYTIYIATASGQFTVLNGSFSLELVSEKYWKVNKPMELYYAPTKEHK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 2
别名 HIP2 interacting protein 3, EC:2.3.2.27, E3 ubiquitin-protein ligase RING2, DING, BAP1
计算分子量 336 aa, 38 kDa
观测分子量 38 kDa
GenBank蛋白编号BC012583
基因名称 RNF2
Gene ID (NCBI) 6045
RRIDAB_2180459
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99496
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNF2 (also known as ding, Ring1B or Ring2) is a member of the Ring finger protein family, which functions as E3 ubiquitin ligase for monoubiquitination of histone H2A at lysine 119 (H2AK119ub). Many studies have demonstrated that overexpressed RNF2 was involved in the pathological progression of multiple cancers and has an impact on their clinical features. For instance, the upregulated expression level of RNF2 is positively correlated with the occurrence and progression of hepatocellular carcinoma, melanoma, prostate cancer, breast cancer, pancreatic cancer, gastric cancer, and bladder urothelial carcinoma, as well as with the radioresistance of lung cancer and chemoresistance of ovarian cancer (PMID: 33400276). The molecular weight of RNF2 is 38 kDa.

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