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RNF181 Polyclonal antibody

RNF181 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 20408-1-AP

Print datasheet

Synonyms

HSPC238, ring finger protein 181, RN181, RNF181

验证数据展示

  • WB analysis of Jurkat using 20408-1-AP

    WB analysis of Jurkat using 20408-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20408-1-AP (RNF181 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 20408-1-AP

    IHC staining of human kidney using 20408-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 20408-1-AP

    IHC staining of human kidney using 20408-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 20408-1-AP

    IF Staining of HepG2 using 20408-1-AP

    Immunofluorescent analysis of HepG2 cells, using RNF181 antibody 20408-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of Jurkat using 20408-1-AP

    IP experiment of Jurkat using 20408-1-AP

    IP result of anti-RNF181 (IP:20408-1-AP, 3ug; Detection:20408-1-AP 1:500) with Jurkat cells lysate 1200ug.

经过测试的应用

Positive WB detected inJurkat cells, L02 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 20408-1-AP is RNF181 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Immunogen RNF181 fusion protein Ag14233 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 181
Synonyms HSPC238, ring finger protein 181, RN181, RNF181
Calculated Molecular Weight 153 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC002803
Gene Symbol RNF181
Gene ID (NCBI) 51255
RRIDAB_10696314
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P0P0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF181, also named as HSPC238, is a 153 amino acid protein, which belongs to the RNF181 family. RNF181 is widely expressed, with highest levels in liver and heart and lowest levels in brain and skeletal muscle. RNF181 as a E3 ubiquitin-protein ligase, accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Western blot analysis detected RNF181 protein in human platelets as a 36-kD dimer and an 18-kD monomer.

实验方案

Product Specific Protocols
WB protocol for RNF181 antibody 20408-1-APDownload protocol
IHC protocol for RNF181 antibody 20408-1-APDownload protocol
IF protocol for RNF181 antibody 20408-1-APDownload protocol
IP protocol for RNF181 antibody 20408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Oncol Rep

Screening and verification of proteins that interact with HSPC238.

Authors - Jia-Yu Tan
View Article