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验证数据展示

  • WB analysis using 30941-1-AP

    WB analysis using 30941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30941-1-AP (RNF180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 30941-1-AP

    IHC staining of mouse kidney using 30941-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30941-1-AP (RNF180 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 30941-1-AP

    IF Staining of A431 using 30941-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RNF180 antibody (30941-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

RNF180 Polyclonal antibody

RNF180 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30941-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

RNF 180, RING finger protein 180, RINES, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF180

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, mouse kindey tissue, mouse liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30941-1-AP targets RNF180 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RNF180 fusion protein Ag34138 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 180
别名 RNF 180, RING finger protein 180, RINES, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF180
计算分子量 592 aa, 68 kDa
观测分子量70 kDa,48kDa
GenBank蛋白编号BC101277
基因名称 RNF180
Gene ID (NCBI) 285671
RRIDAB_3669788
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ86T96
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNF180 antibody 30941-1-APDownload protocol
IHC protocol for RNF180 antibody 30941-1-APDownload protocol
IF protocol for RNF180 antibody 30941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols