验证数据展示
经过测试的应用
Positive WB detected in | SH-SY5Y cells, mouse brain tissue, mouse testis tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 2 publications below |
产品信息
24618-1-AP targets RNF167 in WB, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse |
文献引用反应性 | human |
免疫原 | RNF167 fusion protein Ag18383 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | ring finger protein 167 |
别名 | 5730408C10Rik, LP2254, ring finger protein 167, RING105, RNF167 |
计算分子量 | 350 aa, 38 kDa |
观测分子量 | 46-50 kDa |
GenBank蛋白编号 | BC010139 |
基因名称 | RNF167 |
Gene ID (NCBI) | 26001 |
RRID | AB_2879642 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9H6Y7 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNF167 also named as, ring finger protein 167 or LP2254, is a 350 amino acid protein, which contains one protease associated domain and one Ring-type zinc finger. RNF167 as single-pass membrane protein localizes in the endomembrane system and is strongly expressed in the kidney and liver. RNF167 may act as an E3 ubiquitin-protein ligase,or as a part of the E3 complex, which accepts ubiquitin from specific E3 ubiquitin from ubiquitin conjugation enzymes. RNF167 also may play a role in growth regulation in G1/S transition. The molecular weight of RNF167 is 38 kDa, but we detected a 47 kDa protein by western blot. Our result is similar to the figure of one research paper (PMID:23129617).
实验方案
Product Specific Protocols | |
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WB protocol for RNF167 antibody 24618-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Nat Commun RNF167 activates mTORC1 and promotes tumorigenesis by targeting CASTOR1 for ubiquitination and degradation.
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FASEB J RNF167-mediated ubiquitination of Tollip inhibits TNF-α-triggered NF-κB and MAPK activation |