验证数据展示
经过测试的应用
| Positive WB detected in | MDA-MB-231 cells, mouse brain tissue, mouse testis tissue, SH-SY5Y cells, HeLa cells, MCF-7 cells |
| Positive IF/ICC detected in | HaCaT cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24618-1-AP targets RNF167 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag18383 Product name: Recombinant human RNF167 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-200 aa of BC010139 Sequence: IRATSDHNASMDFADLPALFGATLSQEGLQGFLVEAHPDNACSPIAPPPPAPVNGSVFIALLRRFDCNFDLKVLNAQKAGYGAAVVHNVNSNELLNMVWNSEEIQQQIWIPSVFIGERSSEYLRALFVYEKGARVLLVPDNTFPLGYYLIPFTGIVGLLVLAMGAVMIARCIQHR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ring finger protein 167 |
| 别名 | E3 ubiquitin-protein ligase RNF167, EC:2.3.2.27, LP2254, RING finger protein 167, RING105 |
| 计算分子量 | 350 aa, 38 kDa |
| 观测分子量 | 46-50 kDa |
| GenBank蛋白编号 | BC010139 |
| 基因名称 | RNF167 |
| Gene ID (NCBI) | 26001 |
| RRID | AB_2879642 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H6Y7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNF167 also named as, ring finger protein 167 or LP2254, is a 350 amino acid protein, which contains one protease associated domain and one Ring-type zinc finger. RNF167 as single-pass membrane protein localizes in the endomembrane system and is strongly expressed in the kidney and liver. RNF167 may act as an E3 ubiquitin-protein ligase,or as a part of the E3 complex, which accepts ubiquitin from specific E3 ubiquitin from ubiquitin conjugation enzymes. RNF167 also may play a role in growth regulation in G1/S transition. The molecular weight of RNF167 is 38 kDa, but we detected a 47 kDa protein by western blot. Our result is similar to the figure of one research paper (PMID:23129617).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RNF167 antibody 24618-1-AP | Download protocol |
| WB protocol for RNF167 antibody 24618-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun RNF167 activates mTORC1 and promotes tumorigenesis by targeting CASTOR1 for ubiquitination and degradation.
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FASEB J RNF167-mediated ubiquitination of Tollip inhibits TNF-α-triggered NF-κB and MAPK activation | ||
Nat Commun RNF167 mediates atypical ubiquitylation and degradation of RLRs via two distinct proteolytic pathways
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