验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse kidney tissue, HepG2 cells, rat kidney tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24524-1-AP targets RNF145 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RNF145 fusion protein Ag21463 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ring finger protein 145 |
| 别名 | EC:2.3.2.27, RING finger protein 145 |
| 计算分子量 | 663 aa, 76 kDa |
| 观测分子量 | 76-79 kDa |
| GenBank蛋白编号 | BC042684 |
| 基因名称 | RNF145 |
| Gene ID (NCBI) | 153830 |
| RRID | AB_2879589 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96MT1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF145 antibody 24524-1-AP | Download protocol |
| IHC protocol for RNF145 antibody 24524-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. | ||
EMBO J Regulation of membrane fluidity by RNF145-triggered degradation of the lipid hydrolase ADIPOR2
| ||
World J Surg Oncol Hypoxia-based classification and prognostic signature for clinical management of hepatocellular carcinoma | ||
J Agric Food Chem Methionine and Leucine Promote mTOR Gene Transcription and Milk Synthesis in Mammary Epithelial Cells through the eEF1Bα-UBR5-ARID1A Signaling |
