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验证数据展示

  • WB analysis of MDA-MB-231 using 26144-1-AP (same clone as 26144-1-PBS)

    WB analysis of MDA-MB-231 using 26144-1-AP (same clone as 26144-1-PBS)

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 26144-1-AP (RNF144A antibody) at dilution of 1:1500 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26144-1-PBS in a different storage buffer formulation.

  • IP experiment of CHO using 26144-1-AP (same clone as 26144-1-PBS)

    IP experiment of CHO using 26144-1-AP (same clone as 26144-1-PBS)

    IP result of anti-RNF144A (IP:26144-1-AP, 4ug; Detection:26144-1-AP 1:300) with CHO cells lysate 3100ug. This data was developed using the same antibody clone with 26144-1-PBS in a different storage buffer formulation.

RNF144A Polyclonal antibody, PBS Only

RNF144A Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 26144-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, hamster

应用

WB, IP, Indirect ELISA

别名

UbcM4-interacting protein 4, UBCE7IP4, RNF144, KIAA0161, EC:2.3.2.31

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

26144-1-PBS targets RNF144A in WB, IP, Indirect ELISA applications and shows reactivity with human, hamster samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, hamster
免疫原

CatNo: Ag23508

Product name: Recombinant human RNF144A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 52-179 aa of BC050373

Sequence: ELLIKEGLETAISCPDAACPKQGHLQENEIECMVAAEIMQRYKKLQFEREVLFDPCRTWCPASTCQAVCQLQDVGLQTPQPVQCKACRMEFCSTCKASWHPGQGCPETMPITFLPGETSAAFKMEEDD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 144A
别名 UbcM4-interacting protein 4, UBCE7IP4, RNF144, KIAA0161, EC:2.3.2.31
观测分子量 33 kDa
GenBank蛋白编号BC050373
基因名称 RNF144A
Gene ID (NCBI) 9781
RRIDAB_2880402
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50876
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNF144A (Ring Finger protein 144A) as the first E3 ubiquitin ligase for cytosolic DNA-PKcs, is induced in a p53-dependent manner during DNA damage and targets cytosolic DNA-PKcs for ubiquitination and degradation. RNF144A is an RBR-containing protein, and is unstable and degraded through the proteasome-dependent pathway (PMID: 24979766). RNF144A contains an RBR domain in the N-terminus and a potential single-transmembrane (TM) domain in the C-terminus, and is distributed in both plasma membrane and the membranes of intracellular vesicles including endosomes, lysosomes, endoplasmic reticulum (ER), perinuclear vesicles, and others, which might be caused by the presence of the TM domain (PMID: 37955227).

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