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验证数据展示

  • WB analysis using 83462-1-RR

    WB analysis using 83462-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83462-1-RR (RNF133 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83462-1-RR

    Affinity and Kinetic Characterization of 83462-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83462-1-RR against Human RNF133 were performed. The affinity constant is 0.714 nM.

RNF133 Recombinant antibody

RNF133 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83462-1-RR

产品说明书

CloneNo. 240397C12

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

RING-type E3 ubiquitin transferase RNF133, RING finger protein 133, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF133, 240397C12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83462-1-RR targets RNF133 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 RNF133 fusion protein Ag14537 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ring finger protein 133
别名 RING-type E3 ubiquitin transferase RNF133, RING finger protein 133, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF133, 240397C12
计算分子量 376 aa, 42 kDa
观测分子量47 kDa
GenBank蛋白编号BC022038
基因名称 RNF133
Gene ID (NCBI) 168433
RRIDAB_3671093
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8WVZ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF133 (ring finger protein 133) contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions.

实验方案

Product Specific Protocols
WB protocol for RNF133 antibody 83462-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols