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验证数据展示

  • WB analysis using 25260-1-AP (same clone as 25260-1-PBS)

    WB analysis using 25260-1-AP (same clone as 25260-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25260-1-AP (RNF123 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25260-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 25260-1-AP (same clone as 25260-1-PBS)

    IP experiment of HeLa using 25260-1-AP (same clone as 25260-1-PBS)

    IP result of anti-RNF123 (IP:25260-1-AP, 4ug; Detection:25260-1-AP 1:600) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 25260-1-PBS in a different storage buffer formulation.

RNF123 Polyclonal antibody, PBS Only

RNF123 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 25260-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RNF123 Ready-To-Use IHC Kit. RNF123 primary antibody included.

产品信息

25260-1-PBS targets RNF123 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19653

Product name: Recombinant human RNF123 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 12-358 aa of BC130632

Sequence: RKSYRLTSDAEKSRVTGIVQEKLLNDYLNRIFSSSEHAPPAATSRKPLNFQNLPEHLDQLLQVDNEEEESQGQVEGRLGPSTVVLDHTGGFEGLLLVDDDLLGVIGHSNFGTIRSTTCVYKGKWLYEVLISSQGLMQIGWCTISCRFNQEEGVGDTHNSYAYDGNRVRKWNVTTTNYGKAWAAGDIVSCLIDLDDGTLSFCLNGVSLGTAFENLSRGLGMAYFPAISLSFKESVAFNFGSRPLRYPVAGYRPLQDPPSADLVRAQRLLGCFRAVLSVELDPVEGRLLDKESSKWRLRGQPTVLLTLAHIFHHFAPLLRKVYLVEAVLMSFLLGIVEKGTPTQAQSVV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 123
别名
计算分子量 1314 aa, 149 kDa
观测分子量149 kDa
GenBank蛋白编号BC130632
基因名称 RNF123
Gene ID (NCBI) 63891
RRIDAB_3669467
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5XPI4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RING finger protein 123 (RNF123), also known as KPC1, is an E3 ubiquitin ligase that belongs to the RING-finger family of ligases that contain a 40-60-amino acid long RING domain which is essential for causing substrate ubiquitination (PMID: 29676528). RNF123 promoted TLR3/IRF7-induced type I IFN signaling and inhibited viral replication by degrading SOCS1 (PMID: 37014223).

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