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RNF114 Polyclonal antibody

RNF114 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 14338-1-AP

Print datasheet

Synonyms

ring finger protein 114, RNF114, Zinc finger protein 228, Zinc finger protein 313, ZNF228, ZNF313

验证数据展示

  • WB analysis using 14338-1-AP

    WB analysis using 14338-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14338-1-AP (RNF114 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14338-1-AP targets RNF114 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA
Tested Reactivity human
Immunogen RNF114 fusion protein Ag5649 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 114
Synonyms ring finger protein 114, RNF114, Zinc finger protein 228, Zinc finger protein 313, ZNF228, ZNF313
Calculated Molecular Weight 26 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberBC066919
Gene Symbol RNF114
Gene ID (NCBI) 55905
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y508
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF114, also known as ZNF228 or ZNF313, is a member of the zinc-binding protein family. RNF114 contains two C2H2 and Ring finger zinc-binding domains and a ubiquitin-interacting motif (UIM). RNF114 functions as an E3 ligase in the ubiquitin-proteasome system and plays an important role in basic cellular processes in different types of cells. RNF114 is expressed widely in many normal tissues, such as the heart, liver, and kidney. RNF114 has 2 isoforms with the molecular mass of 21 and 26 kDa. RNF114 is a novel determinant of psoriasis susceptibility, and its overexpression is a crucial indicator of epithelial inflammation (PMID: 34104941, 35069903, 37523804).

实验方案

Product Specific Protocols
WB protocol for RNF114 antibody 14338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols