验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, MCF-7 cells, HEK-293T cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27018-1-AP targets RNF113A in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag25313 Product name: Recombinant human RNF113A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 41-100 aa of BC000832 Sequence: GESGSSSDEGCTVVRPEKKRVTHNPMIQKTRDSGKQKAAYGDLSSEEEEENEPESLGVVY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ring finger protein 113A |
| 别名 | ZNF183, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF113A, Cwc24 homolog |
| 计算分子量 | 39 kDa |
| 观测分子量 | 40-50 kDa |
| GenBank蛋白编号 | BC000832 |
| 基因名称 | RNF113A |
| Gene ID (NCBI) | 7737 |
| RRID | AB_2880724 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15541 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF113A antibody 27018-1-AP | Download protocol |
| IHC protocol for RNF113A antibody 27018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomark Res EIF4A3-induced Circ_0001187 facilitates AML suppression through promoting ubiquitin-proteasomal degradation of METTL3 and decreasing m6A modification level mediated by miR-499a-5p/RNF113A pathway | ||
Am J Transl Res Prognostic value of RNF113A shows a correlation with immune infiltrates in colorectal cancer |