验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25165-1-AP targets RNF112 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag18306 Product name: Recombinant human RNF112 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 287-631 aa of BC053989 Sequence: DLDYLEMFVHVAEVMGKHYGMVPIQHLDLLVRDSSHPNKAGQGHVGNIFQRLSGRYPKVQELLQGKRARCCLLPAPGRRRMNQGHASPGDTDDDFRHLLGAYVSDVLSAAPQHAKSRCQGYWNEGRAVARGDRRLLTGQQLAQEIKNLSGWMGRTGPGFTSPDEMAAQLHDLRKVEAAKREFEEYVRQQDVATKRIFSALRVLPDTMRNLLSTQKDAILARHGVALLCKGRDQTLEALEAELQATAKAFMDSYTMRFCGHLAAVGGAVGAGLMGLAGGVVGAGMAAAALAAEAGMVAAGAAVGATGAAVVGGGVGAGLAATVGCMEKEEDERLLEGDREPLLQEE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ring finger protein 112 |
| 别名 | Brain finger protein, EC:2.3.2.27, Zinc finger protein 179, ZNF179 |
| 计算分子量 | 631 aa, 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC053989 |
| 基因名称 | RNF112 |
| Gene ID (NCBI) | 7732 |
| RRID | AB_2879935 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9ULX5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ring Finger Protein 112 (RNF112), also known as Zinc Finger Protein 179 (ZNF179) or neurolastin, is a protein that is primarily expressed in the brain. RNF112 possesses an autoubiquitination feature and also directly ubiquitinates TDP-43 and Forkhead box M1 (FOXM1) to attenuate related neuropathy and inhibit gastric cancer progression, respectively (PMID: 40178292, 40198702). As reported, RNF112 knockout mice exhibited impairment of brain functions, such as motor balance, spatial learning and memory (PMID: 30454900).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF112 antibody 25165-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
JCI Insight RNF112-mediated FOXM1 ubiquitination suppresses the proliferation and invasion of gastric cancer | ||
Sci Adv KRAS(G12V)/HLA-A*02:01-targeted chimeric antigen receptor T cells exhibit potent preclinical activity against solid tumors. |
