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验证数据展示

  • WB analysis of Jurkat using 86019-1-RR

    WB analysis of Jurkat using 86019-1-RR

    Jurkat cells were subjected to SDS PAGE followed by western blot with 86019-1-RR (RNF10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 86019-1-RR

    WB analysis of PC-3 using 86019-1-RR

    PC-3 cells were subjected to SDS PAGE followed by western blot with 86019-1-RR (RNF10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of K562 using 86019-1-RR

    IF Staining of K562 using 86019-1-RR

    Immunofluorescent analysis of (4% PFA) fixed K562 using RNF10 antibody (86019-1-RR, Clone: 250438C11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RNF10 Recombinant antibody

RNF10 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86019-1-RR

产品说明书

CloneNo. 250438C11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

E3 ubiquitin-protein ligase RNF10, EC:2.3.2.27, KIAA0262, RIE2, RING finger protein 10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, fetal human brain tissue, PC-3 cells
Positive IF/ICC detected inK562
Planning an IHC experiment? We recommend our IHCeasy RNF10 Ready-To-Use IHC Kit. RNF10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86019-1-RR targets RNF10 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 RNF10 fusion protein Ag10433 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ring finger protein 10
别名 E3 ubiquitin-protein ligase RNF10, EC:2.3.2.27, KIAA0262, RIE2, RING finger protein 10
计算分子量 811 aa, 90 kDa
观测分子量 90 kDa
GenBank蛋白编号BC016622
基因名称 RNF10
Gene ID (NCBI) 9921
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N5U6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. RNF10 (RING finger protein 10), a 90-kDa protein, contains a ring finger motif which is known to be involved in protein-protein interactions. RNF10 regulates myelin-associated glycoprotein (MAG) expression and is required for myelin formation, therefore, participates in the peripheral nerve development and Schawnn cell differentiation. RNF10 is also involved in activation of p21WAF1 promoter via its activity as transcriptional factor.

实验方案

Product Specific Protocols
WB protocol for RNF10 antibody 86019-1-RRDownload protocol
IF protocol for RNF10 antibody 86019-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols