验证数据展示
经过测试的应用
| Positive WB detected in | mouse uterus tissue, PC-3 cells |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13844-1-AP targets RND2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4807 Product name: Recombinant human RND2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 59-227 aa of BC018096 Sequence: MWDTSGSSYYDNVRPLAYPDSDAVLICFDISRPETLDSVLKKWQGETQEFCPNAKVVLVGCKLDMRTDLATLRELSKQRLIPVTHEQGTVLAKQVGAVSYVECSSRSSERSVRDVFHVATVASLGRGHRQLRRTDSRRGMQRSAQLSGRPDRGNEGEIHKDRAKSCNLM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rho family GTPase 2 |
| 别名 | RND 2, Rho-related GTP-binding protein RhoN, Rho-related GTP-binding protein Rho7, Rho family GTPase 2, ARHN |
| 计算分子量 | 227 aa, 25 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC018096 |
| 基因名称 | RND2 |
| Gene ID (NCBI) | 8153 |
| RRID | AB_2181817 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P52198 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RND2 antibody 13844-1-AP | Download protocol |
| IP protocol for RND2 antibody 13844-1-AP | Download protocol |
| WB protocol for RND2 antibody 13844-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Sema3E-Plexin D1 signaling drives human cancer cell invasiveness and metastatic spreading in mice.
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Mol Psychiatry The atypical Rho GTPase Rnd2 is critical for dentate granule neuron development and anxiety-like behavior during adult but not neonatal neurogenesis.
| ||
J Exp Clin Cancer Res RND2 attenuates apoptosis and autophagy in glioblastoma cells by targeting the p38 MAPK signalling pathway.
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Mol Biol Cell Rnd2 differentially regulates oligodendrocyte myelination at different developmental periods
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