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验证数据展示

  • WB analysis using 82771-1-RR (same clone as 82771-1-PBS)

    WB analysis using 82771-1-RR (same clone as 82771-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82771-1-RR (RNASEH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82771-1-RR (same clone as 82771-1-PBS)

    WB analysis of HeLa using 82771-1-RR (same clone as 82771-1-PBS)

    WB result of RNASEH1 antibody (82771-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNASEH1 transfected HeLa cells. This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82771-1-RR (same clone as 82771-1-PBS)

    WB analysis of HeLa using 82771-1-RR (same clone as 82771-1-PBS)

    WB result of RNASEH1 antibody (82771-1-RR; 1:15000; room temperature for 1.5 hours) with wild-type and RNASEH1 knockout HeLa cells. This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 82771-1-RR (same clone as 82771-1-PBS)

    IHC staining of human ovarian cancer using 82771-1-RR (same clone as 82771-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82771-1-RR (RNASEH1 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82771-1-RR

    Affinity and Kinetic Characterization of 82771-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82771-1-RR against Human RNASEH1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证
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RNASEH1 Recombinant monoclonal antibody, PBS Only

RNASEH1 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82771-1-PBS

产品说明书

CloneNo. 2C12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82771-1-PBS targets RNASEH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7990

Product name: Recombinant human RNASEH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-286 aa of BC002973

Sequence: MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribonuclease H1
别名 RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C12
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC002973
基因名称 RNASEH1
Gene ID (NCBI) 246243
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO60930
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].