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验证数据展示

  • WB analysis using 17203-1-AP

    WB analysis using 17203-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17203-1-AP (RNASE11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 17203-1-AP

    IHC staining of human gliomas using 17203-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17203-1-AP (RNASE11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 17203-1-AP

    IHC staining of human gliomas using 17203-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17203-1-AP (RNASE11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RNASE11 Polyclonal antibody

RNASE11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17203-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C14orf6, Probable ribonuclease 11, RNase 11, RNASE11

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inL02 cells, human testis tissue, HepG2 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17203-1-AP targets RNASE11 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10981

Product name: Recombinant human RNASE11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-199 aa of BC025410

Sequence: METFPLLLLSLGLVLAEASESTMKIIKEEFTDEEMQYDMAKSGQEKQTIEILMNPILLVKNTSLSMSKDDMSSTLLTFRSLHYNDPKGNSSGNDKECCNDMTVWRKVSEANGSCKWSNNFIRSSTEVMRRVHRAPSCKFVQNPGISCCESLELENTVCQFTTGKQFPRCQYHSVTSLEKILTVLTGHSLMSWLVCGSKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribonuclease, RNase A family, 11 (non-active)
别名 C14orf6, Probable ribonuclease 11, RNase 11, RNASE11
计算分子量 199 aa, 22 kDa
观测分子量 30 kDa
GenBank蛋白编号BC025410
基因名称 RNASE11
Gene ID (NCBI) 122651
RRIDAB_2253967
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TAA1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNASE11, also named as C14ORF6, is ribonuclease 11. This antibody detected 30 kDa band with Testis and L02 cell lysate.

实验方案

Product Specific Protocols
IHC protocol for RNASE11 antibody 17203-1-APDownload protocol
WB protocol for RNASE11 antibody 17203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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