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验证数据展示

  • WB analysis of A549 using 25803-1-AP

    WB analysis of A549 using 25803-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25803-1-AP (RMND5B Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IHC staining of human colon using 25803-1-AP

    IHC staining of human colon using 25803-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25803-1-AP (RMND5B Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 25803-1-AP

    IHC staining of human colon using 25803-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25803-1-AP (RMND5B Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A549 using 25803-1-AP

    IF Staining of A549 using 25803-1-AP

    Immunofluorescent analysis of A549 cells using 25803-1-AP (RMND5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

RMND5B Polyclonal antibody

RMND5B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25803-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Protein RMD5 homolog B, RMND5B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inhuman colon tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25803-1-AP targets RMND5B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag22714

Product name: Recombinant human RMND5B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-49 aa of BC009911

Sequence: MEQCACVERELDKVLQKFLTYGQHCERSLEELLHYVGQLRAELASAALQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 required for meiotic nuclear division 5 homolog B (S. cerevisiae)
别名 Protein RMD5 homolog B, RMND5B
计算分子量 393 aa, 44 kDa
观测分子量 40-44 kDa
GenBank蛋白编号BC009911
基因名称 RMND5B
Gene ID (NCBI) 64777
RRIDAB_2880243
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96G75
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for RMND5B antibody 25803-1-APDownload protocol
IHC protocol for RMND5B antibody 25803-1-APDownload protocol
WB protocol for RMND5B antibody 25803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway

Authors - Sang Ah Yi
  • KO Validated
View Article
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