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验证数据展示

  • WB analysis using 85697-1-RR

    WB analysis using 85697-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85697-1-RR (RLBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of ARPE-19 using 85697-1-RR

    WB analysis of ARPE-19 using 85697-1-RR

    ARPE-19 cells were subjected to SDS PAGE followed by western blot with 85697-1-RR (RLBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 85697-1-RR

    IHC staining of mouse eye using 85697-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85697-1-RR (RLBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse eye using 85697-1-RR

    IHC staining of mouse eye using 85697-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85697-1-RR (RLBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 85697-1-RR

    IF Staining of mouse eye using 85697-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RLBP1 antibody (85697-1-RR, Clone: 242989D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RLBP1 Recombinant antibody

RLBP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 85697-1-RR

产品说明书

CloneNo. 242989D1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Cellular retinaldehyde-binding protein, CRALBP, Retinaldehyde-binding protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse eye tissue, ARPE-19 cells, mouse retina tissue, rat retina tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85697-1-RR targets RLBP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RLBP1 fusion protein Ag7602 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 retinaldehyde binding protein 1
别名 Cellular retinaldehyde-binding protein, CRALBP, Retinaldehyde-binding protein 1
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC004199
基因名称 RLBP1
Gene ID (NCBI) 6017
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12271
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RLBP1 (Retinaldehyde-binding protein 1) is also named as cellular retinaldehyde-binding protein (CRALBP). CRALBP, a 36-kDa aqueous soluble carrier, is expressed in abundance by both retinal pigment epithelial (RPE) and Muller cells of retina and is important for rod- and cone-driven vision (PMID: 32188692). Retinal RLBP1 expression was decreased in diabetes, and its overexpression in Müller glia mitigated DR-associated neurovascular degeneration (PMID: 33674409).

实验方案

Product Specific Protocols
WB protocol for RLBP1 antibody 85697-1-RRDownload protocol
IHC protocol for RLBP1 antibody 85697-1-RRDownload protocol
IF protocol for RLBP1 antibody 85697-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols