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验证数据展示

  • WB analysis using 84520-1-RR (same clone as 84520-1-PBS)

    WB analysis using 84520-1-RR (same clone as 84520-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84520-1-RR (RIT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84520-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01369-1

    Cytometric bead array standard curve of MP01369-1

    Cytometric bead array standard curve of MP01369-1, RIT2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84520-2-PBS. Detection antibody: 84520-1-PBS. Standard: Ag3160. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84520-1-RR

    Affinity and Kinetic Characterization of 84520-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84520-1-RR against Human RIT2 were performed. The affinity constant is below 1 pM.

RIT2 Recombinant antibody, PBS Only (Detector)

RIT2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 84520-1-PBS

产品说明书

CloneNo. 241916A7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

Ras like without CAAX protein 2, Ras like protein expressed in neurons, GTP-binding protein Rit2, GTP binding protein Rit2, EC:3.6.5.2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84520-1-PBS targets RIT2 as part of a matched antibody pair:

MP01369-1: 84520-2-PBS capture and 84520-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 RIT2 fusion protein Ag3160 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Ras-like without CAAX 2
别名 Ras like without CAAX protein 2, Ras like protein expressed in neurons, GTP-binding protein Rit2, GTP binding protein Rit2, EC:3.6.5.2
计算分子量 217 aa, 25 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC018060
基因名称 RIT2
Gene ID (NCBI) 6014
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99578
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RIT2 (AKA: Rin, Ras-like in neurons) is a small, neuronal, ras-like GTPase with enriched expression in SNc DANs (PMID: 38395968). RIT2 is a member of the Ras superfamily that plays important roles in many vital cellular functions, such as differentiation and survival (PMID: 29860660). Rit2 directly interacts with the DA transporter (DAT), and is required for regulated DAT membrane trafficking. In cell culture models, Rit2 is required for EGF- and NGF-mediated neurite outgrowth, NGF-mediated ERK phosphorylation, and cell viability (PMID: 38395968).