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验证数据展示

  • WB analysis using 26977-1-AP

    WB analysis using 26977-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26977-1-AP (RIT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 26977-1-AP

    IF Staining of MCF-7 using 26977-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RIT1 antibody (26977-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RIT1 Polyclonal antibody

RIT1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26977-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

GTP binding protein Rit1, Ras like protein expressed in many tissues, Ras like without CAAX 1, Ras like without CAAX protein 1, RIBB

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, human placenta tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26977-1-AP targets RIT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag25636

Product name: Recombinant human RIT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 140-219 aa of BC104186

Sequence: QLRQVTKEEGLALAREFSCPFFETSAAYRYYIDDVFHALVREIRRKEKEAVLAMEKKSKPKNSVWKRLKSPFRKKKDSVT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ras-like without CAAX 1
别名 GTP binding protein Rit1, Ras like protein expressed in many tissues, Ras like without CAAX 1, Ras like without CAAX protein 1, RIBB
计算分子量 25 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC104186
基因名称 RIT1
Gene ID (NCBI) 6016
RRIDAB_3669578
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92963
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIT1 (Ras-like without CAAX protein 1) is also named as RIBB, ROC1 and GTP-binding protein Rit1. RIT1 serves as a regulatory factor for neuronal cell proliferation, survival, and differentiation (PMID: 28007959). Rit1 mediates oxidative stress resistance, contributing to cell survival via the p38 MAPK signaling pathway (PMID: 21737674). As a small G protein of Ras family, RIT1 plays a critical role in various tumors (such as hepatocellular carcinoma, HCC) (PMID: 38017479). It is worth noting that RIT1 is frequently amplified in various human cancers including HCC, lung adenocarcinoma, cholangiocarcinoma, uterine carcinosarcoma, breast cancer, and ovarian cancer (PMID: 38017479).

实验方案

Product Specific Protocols
IF protocol for RIT1 antibody 26977-1-APDownload protocol
WB protocol for RIT1 antibody 26977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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