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验证数据展示

  • WB analysis using 13593-1-AP (same clone as 13593-1-PBS)

    WB analysis using 13593-1-AP (same clone as 13593-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 13593-1-AP (same clone as 13593-1-PBS)

    IHC staining of human breast cancer using 13593-1-AP (same clone as 13593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13593-1-AP (RIOK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 13593-1-AP (same clone as 13593-1-PBS)

    IHC staining of human breast cancer using 13593-1-AP (same clone as 13593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13593-1-AP (RIOK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 13593-1-AP (same clone as 13593-1-PBS)

    IP experiment of mouse testis using 13593-1-AP (same clone as 13593-1-PBS)

    IP result of anti-RIOK3 (IP:13593-1-AP, 4ug; Detection:13593-1-AP 1:600) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 13593-1-AP (same clone as 13593-1-PBS)

    IF Staining of MCF-7 using 13593-1-AP (same clone as 13593-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RIOK3 antibody (13593-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 13593-1-AP (same clone as 13593-1-PBS)

    IF Staining of A431 using 13593-1-AP (same clone as 13593-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RIOK3 antibody (13593-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RIOK3 Polyclonal antibody, PBS Only

RIOK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13593-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

sudD homolog, SUDD, Serine/threonine-protein kinase RIO3, RIO kinase 3 (yeast), RIO kinase 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13593-1-PBS targets RIOK3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4497

Product name: Recombinant human RIOK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 215-516 aa of BC039729

Sequence: RRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RIO kinase 3 (yeast)
别名 sudD homolog, SUDD, Serine/threonine-protein kinase RIO3, RIO kinase 3 (yeast), RIO kinase 3
计算分子量 516 aa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC039729
基因名称 RIOK3
Gene ID (NCBI) 8780
RRIDAB_2178105
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14730
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RIOK3(Serine/threonine-protein kinase RIO3) is initially characterized as a homolog of Aspergillus nidulans sudD and showed down-regulation at the invasive front of malignant melanomas.On Western blots, human RIOK3 migrated as a 60-kD protein. Northern blot analysis indicated that the approximately 4-kb RIOK3 mRNA is expressed widely in human tissues, with additional smaller transcripts present in testis.

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