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RIOK1 Polyclonal antibody

RIOK1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 17222-1-AP

Print datasheet

Synonyms

AD034, RIO kinase 1, RIO kinase 1 (yeast), RIOK1

验证数据展示

  • WB analysis of HeLa using 17222-1-AP

    WB analysis of HeLa using 17222-1-AP

    WB result of RIOK1 antibody (17222-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RIOK1 transfected HeLa cells.

  • WB analysis of HeLa using 17222-1-AP

    WB analysis of HeLa using 17222-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17222-1-AP (RIOK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 17222-1-AP

    IP experiment of HeLa using 17222-1-AP

    IP result of anti-RIOK1 (IP:17222-1-AP, 4ug; Detection:17222-1-AP 1:500) with HeLa cells lysate 3200ug.

  • WB analysis using 17222-1-AP

    WB analysis using 17222-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17222-1-AP (RIOK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

17222-1-AP targets RIOK1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RIOK1 fusion protein Ag11057 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RIO kinase 1 (yeast)
Synonyms AD034, RIO kinase 1, RIO kinase 1 (yeast), RIOK1
Calculated Molecular Weight 568 aa, 66 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC006104
Gene Symbol RIOK1
Gene ID (NCBI) 83732
RRIDAB_2284990
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRS2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RIOK1 antibody 17222-1-APDownload protocol
IP protocol for RIOK1 antibody 17222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biomedicines

RIOK2 Inhibitor NSC139021 Exerts Anti-Tumor Effects on Glioblastoma via Inducing Skp2-Mediated Cell Cycle Arrest and Apoptosis.

Authors - Min Yu
View Article
humanWB

iScience

Ras Post-transcriptionally Enhances a Pre-malignantly Primed EMT to Promote Invasion.

Authors - Laura S Bisogno
View Article
humanWB

Cancer Res Treat

Elevated Expression of RIOK1 Is Correlated with Breast Cancer Hormone Receptor Status and Promotes Cancer Progression.

Authors - Zhiqi Huang
View Article