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RIN1 Polyclonal antibody

RIN1 Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 16388-1-AP

Print datasheet

Synonyms

Ras and Rab interactor 1, Ras inhibitor JC99, RIN1

验证数据展示

  • WB analysis of HeLa using 16388-1-AP

    WB analysis of HeLa using 16388-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16388-1-AP (RIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16388-1-AP

    IP experiment of HeLa using 16388-1-AP

    IP result of anti-RIN1 (IP:16388-1-AP, 4ug; Detection:16388-1-AP 1:500) with HeLa cells lysate 3400ug.

  • IF Staining of HeLa using 16388-1-AP

    IF Staining of HeLa using 16388-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16388-1-AP (RIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16388-1-AP targets RIN1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RIN1 fusion protein Ag9451 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Ras and Rab interactor 1
Synonyms Ras and Rab interactor 1, Ras inhibitor JC99, RIN1
Calculated Molecular Weight 783 aa, 84 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC014417
Gene Symbol RIN1
Gene ID (NCBI) 9610
RRIDAB_2177839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13671
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RIN1 antibody 16388-1-APDownload protocol
IF protocol for RIN1 antibody 16388-1-APDownload protocol
IP protocol for RIN1 antibody 16388-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Comput Struct Biotechnol J

Structure-based discovery of neoandrographolide as a novel inhibitor of Rab5 to suppress cancer growth.

Authors - Jing Zhang
View Article
humanIF

Cell Commun Signal

Circular dorsal ruffles disturb the growth factor-induced PI3K-AKT pathway in hepatocellular carcinoma Hep3B cells.

Authors - Xiaowei Sun
View Article