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  • Featured Product
  • KD/KO Validated

RIMKLB Polyclonal antibody

RIMKLB Polyclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26111-1-AP

Print datasheet

Synonyms

FAM80B, KIAA1238, RIMKLB

验证数据展示

  • IHC staining of mouse testis using 26111-1-AP

    IHC staining of mouse testis using 26111-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26111-1-AP (RIMKLB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 26111-1-AP

    IHC staining of mouse testis using 26111-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26111-1-AP (RIMKLB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse eye using 26111-1-AP

    WB analysis of mouse eye using 26111-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 26111-1-AP (RIMKLB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 26111-1-AP

    IF Staining of mouse testis using 26111-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using RIMKLB antibody (26111-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse testis using 26111-1-AP

    IF Staining of mouse testis using 26111-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using RIMKLB antibody (26111-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse eye tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26111-1-AP is RIMKLB Fusion Protein expressed in E. coli.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen RIMKLB fusion protein Ag23631 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal modification protein rimK-like family member B
Synonyms FAM80B, KIAA1238, RIMKLB
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42-48 kDa
GenBank Accession NumberBC033793
Gene Symbol RIMKLB
Gene ID (NCBI) 57494
RRIDAB_2880383
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULI2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RIMKLB antibody 26111-1-APDownload protocol
IHC protocol for RIMKLB antibody 26111-1-APDownload protocol
IF protocol for RIMKLB antibody 26111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

Comprehensive metabolomics expands precision medicine for triple-negative breast cancer.

Authors - Yi Xiao
  • KD Validated
View Article
WB

J Inherit Metab Dis

NAAG synthetase deficiency has only low influence on pathogenesis in a Canavan disease mouse model

Authors - Ivonne Becker
View Article