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验证数据展示

  • WB analysis using 24576-1-AP (same clone as 24576-1-PBS)

    WB analysis using 24576-1-AP (same clone as 24576-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24576-1-AP (RIM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24576-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 24576-1-AP (same clone as 24576-1-PBS)

    IHC staining of mouse brain using 24576-1-AP (same clone as 24576-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24576-1-AP (RIM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24576-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 24576-1-AP (same clone as 24576-1-PBS)

    IHC staining of mouse brain using 24576-1-AP (same clone as 24576-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24576-1-AP (RIM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24576-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 24576-1-AP (same clone as 24576-1-PBS)

    IP experiment of mouse brain using 24576-1-AP (same clone as 24576-1-PBS)

    IP result of anti-RIM1 (IP:24576-1-AP, 4ug; Detection:24576-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 24576-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RIM1 Polyclonal antibody, PBS Only

RIM1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 24576-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24576-1-PBS targets RIM1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag20161

Product name: Recombinant human RIMS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 923-1039 aa of BC152435

Sequence: KSTTLTVPEQQRTTHHRSRSVSPHRGNDQGKPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPVDHRTRDVDSQYLSEQDSELLMLPRAKRGRSAECLHTTR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulating synaptic membrane exocytosis 1
别名
计算分子量 1692 aa, 189 kDa
观测分子量 189 kDa
GenBank蛋白编号BC152435
基因名称 RIMS1
Gene ID (NCBI) 22999
RRIDAB_2879618
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UR5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RIMS1 is a RAS gene superfamily member that regulates synaptic vesicle exocytosis. RIMS1 also plays a role in the regulation of voltage-gated calcium channels during neurotransmitter and insulin release. Mutations in RIMS1 have suggested a role cognition and have been identified as the cause of cone-rod dystrophy type 7.

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