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验证数据展示

  • WB analysis using 82996-4-RR

    WB analysis using 82996-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82996-4-RR (RILP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 82996-4-RR

    WB analysis of HepG2 using 82996-4-RR

    WB result of RILP antibody (82996-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RILP transfected HepG2 cells.

  • IHC staining of human ovarian cancer using 82996-4-RR

    IHC staining of human ovarian cancer using 82996-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82996-4-RR (RILP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 82996-4-RR

    FC experiment of HeLa using 82996-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RILP Recombinant antibody (82996-4-RR, Clone:230208G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82996-4-RR

    Affinity and Kinetic Characterization of 82996-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82996-4-RR against Human IGFBP4 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

RILP Recombinant antibody

RILP Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 82996-4-RR

产品说明书

CloneNo. 230208G2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

230208G2, FLJ31193, PP10141, Rab-interacting lysosomal protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82996-4-RR targets RILP in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 RILP fusion protein Ag4472 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
亲和力
KD=1.04 x 10-11M
KOff=2.33 x 10-5M
KOn=2.05 x 106M
全称 Rab interacting lysosomal protein
别名 230208G2, FLJ31193, PP10141, Rab-interacting lysosomal protein
计算分子量 401 aa, 41 kDa
观测分子量 50 kDa
GenBank蛋白编号BC031621
基因名称 RILP
Gene ID (NCBI) 83547
RRIDAB_3670743
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96NA2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RILP antibody 82996-4-RRDownload protocol
IHC protocol for RILP antibody 82996-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols