验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
15024-1-AP targets ARHGAP32 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag7104 Product name: Recombinant human RICS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 5-322 aa of BC000277 Sequence: VLSTWWRGKHGFQVGLFPGHCVELINQKVPQSVTNSVPKPVSKKHGKLITFLRTFMKSRPTKQKLKQRGILKERVFGCDLGEHLLNSGFEVPQVLQSCTAFIERYGIVDGIYRLSGVASNIQRLRHEFDSEHVPDLTKEPYVQDIHSVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSLLADYCSITNMHAKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLLPHFSARTELIVPFPLRLLRKQFTPPLLGPMSPLNPLVQITVCISI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rho GTPase-activating protein |
| 别名 | ARHGAP32, GC GAP, GRIT, KIAA0712, p200RhoGAP, p250GAP, Rho GTPase activating protein, RICS |
| 计算分子量 | 231 kDa |
| 观测分子量 | 220 kDa |
| GenBank蛋白编号 | BC000277 |
| 基因名称 | ARHGAP32 |
| Gene ID (NCBI) | 9743 |
| RRID | AB_3085448 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | A7KAX9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARHGAP32, also known as RICS, belongs to the PX domain-containing GAP family. ARHGAP32 encodes a neuron-associated GTPase-activating protein that regulates dendritic spine morphology and strength. ARHGAP32 also can interact with RhoA and β-catenin, thereby inhibiting phosphorylation of LIMK/cofilin and promoting epithelial-to-mesenchymal transition (EMT) process. ARHGAP32 is highly expressed in brain and testis (PMID: 30888095, PMID: 32205841).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARHGAP32 antibody 15024-1-AP | Download protocol |
| IHC protocol for ARHGAP32 antibody 15024-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
