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验证数据展示

  • WB analysis using 15024-1-AP

    WB analysis using 15024-1-AP

    HeLa cells were subjected to Tris-Acetate gel system followed by western blot with 15024-1-AP (ARHGAP32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 15024-1-AP

    IHC staining of mouse testis using 15024-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15024-1-AP (ARHGAP32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ARHGAP32 Polyclonal antibody

ARHGAP32 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15024-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

ARHGAP32, GC GAP, GRIT, KIAA0712, p200RhoGAP, p250GAP, Rho GTPase activating protein, RICS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15024-1-AP targets ARHGAP32 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 ARHGAP32 fusion protein Ag7104 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho GTPase-activating protein
别名 ARHGAP32, GC GAP, GRIT, KIAA0712, p200RhoGAP, p250GAP, Rho GTPase activating protein, RICS
计算分子量 231 kDa
观测分子量220 kDa
GenBank蛋白编号BC000277
基因名称 ARHGAP32
Gene ID (NCBI) 9743
RRIDAB_3085448
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA7KAX9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARHGAP32, also known as RICS, belongs to the PX domain-containing GAP family. ARHGAP32 encodes a neuron-associated GTPase-activating protein that regulates dendritic spine morphology and strength. ARHGAP32 also can interact with RhoA and β-catenin, thereby inhibiting phosphorylation of LIMK/cofilin and promoting epithelial-to-mesenchymal transition (EMT) process. ARHGAP32 is highly expressed in brain and testis (PMID: 30888095, PMID: 32205841).

实验方案

Product Specific Protocols
WB protocol for ARHGAP32 antibody 15024-1-APDownload protocol
IHC protocol for ARHGAP32 antibody 15024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Nutr Biochem

Hippocampal proteomic changes in high-fat diet-induced obese mice associated with memory decline

Authors - Ping Lu
View Article