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ARHGAP32 Polyclonal antibody

ARHGAP32 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15024-1-AP

Print datasheet

Synonyms

ARHGAP32, GC GAP, GRIT, KIAA0712, p200RhoGAP, p250GAP, Rho GTPase activating protein, RICS

验证数据展示

  • WB analysis using 15024-1-AP

    WB analysis using 15024-1-AP

    HeLa cells were subjected to Tris-Acetate gel system followed by western blot with 15024-1-AP (ARHGAP32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 15024-1-AP

    IHC staining of mouse testis using 15024-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15024-1-AP (ARHGAP32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15024-1-AP targets ARHGAP32 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen ARHGAP32 fusion protein Ag7104 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho GTPase-activating protein
Synonyms ARHGAP32, GC GAP, GRIT, KIAA0712, p200RhoGAP, p250GAP, Rho GTPase activating protein, RICS
Calculated Molecular Weight 231 kDa
Observed Molecular Weight220 kDa
GenBank Accession NumberBC000277
Gene Symbol RICS
Gene ID (NCBI) 9743
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA7KAX9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARHGAP32, also known as RICS, belongs to the PX domain-containing GAP family. ARHGAP32 encodes a neuron-associated GTPase-activating protein that regulates dendritic spine morphology and strength. ARHGAP32 also can interact with RhoA and β-catenin, thereby inhibiting phosphorylation of LIMK/cofilin and promoting epithelial-to-mesenchymal transition (EMT) process. ARHGAP32 is highly expressed in brain and testis (PMID: 30888095, PMID: 32205841).

实验方案

Product Specific Protocols
WB protocol for ARHGAP32 antibody 15024-1-APDownload protocol
IHC protocol for ARHGAP32 antibody 15024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Nutr Biochem

Hippocampal proteomic changes in high-fat diet-induced obese mice associated with memory decline

Authors - Ping Lu
View Article