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验证数据展示

  • WB analysis of HeLa using 66625-1-Ig (same clone as 66625-1-PBS)

    WB analysis of HeLa using 66625-1-Ig (same clone as 66625-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66625-1-Ig (RIC8A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66625-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 66625-1-Ig (same clone as 66625-1-PBS)

    IP experiment of HEK-293 using 66625-1-Ig (same clone as 66625-1-PBS)

    IP result of anti-RIC8A (IP:66625-1-Ig, 5ug; Detection:66625-1-Ig 1:2000) with HEK-293 cells lysate 4000 ug. This data was developed using the same antibody clone with 66625-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66625-1-Ig (same clone as 66625-1-PBS)

    IHC staining of mouse brain using 66625-1-Ig (same clone as 66625-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66625-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RIC8A antibody included
  • IHC staining of mouse brain using 66625-1-Ig (same clone as 66625-1-PBS)

    IHC staining of mouse brain using 66625-1-Ig (same clone as 66625-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66625-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RIC8A antibody included
  • IF Staining of mouse brain using 66625-1-Ig (same clone as 66625-1-PBS)

    IF Staining of mouse brain using 66625-1-Ig (same clone as 66625-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RIC8A antibody (66625-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66625-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66625-1-Ig (same clone as 66625-1-PBS)

    IF Staining of mouse brain using 66625-1-Ig (same clone as 66625-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RIC8A antibody (66625-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66625-1-PBS in a different storage buffer formulation.

RIC8A Monoclonal antibody, PBS Only

RIC8A Monoclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 66625-1-PBS

产品说明书

CloneNo. 1E3B11

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat, Mouse

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

Protein Ric 8A, RIC8, RIC8A, synembryn, Synembryn A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RIC8A Ready-To-Use IHC Kit. RIC8A primary antibody included.

产品信息

66625-1-PBS targets RIC8A in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 Human, Rat, Mouse
免疫原

CatNo: Ag1593

Product name: Recombinant human RIC8A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 117-467 aa of BC011821

Sequence: QQLFQELKGVRLLTDTLELTLGVTPEGNPPTLLPSQETERAMEILKVLFNITLDSIKGEVDEEDAALYRHLGTLLRHCVMIATAGDRTEEFHGHAVNLLGNLPLKCLDVLLTLEPHGDSTEFMGVNMDVIRALLIFLEKRLHKTHRLKESVAPVLSVLTECARMHRPARKFLKAQVLPPLRDVRTRPEVGEMLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLAARGLMAGGRPEGQYSEDEDTDTDEYKEAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAMKLVTMFDKLSRNRVIQPMGMSPRGHLTSLQDAMCETMEQQLSSDPDSDPD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
别名 Protein Ric 8A, RIC8, RIC8A, synembryn, Synembryn A
计算分子量 60 kDa
观测分子量 65 kDa
GenBank蛋白编号BC011821
基因名称 RIC8A
Gene ID (NCBI) 60626
RRIDAB_2881985
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9NPQ8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.

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