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验证数据展示

  • WB analysis using 15272-1-AP

    WB analysis using 15272-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15272-1-AP (RIBC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 15272-1-AP

    WB analysis of human brain using 15272-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15272-1-AP (RIBC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 15272-1-AP

    IHC staining of human cervical cancer using 15272-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15272-1-AP (RIBC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RIBC2 antibody included
  • IHC staining of mouse testis using 15272-1-AP

    IHC staining of mouse testis using 15272-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15272-1-AP (RIBC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RIBC2 antibody included
  • IF Staining of HeLa using 15272-1-AP

    IF Staining of HeLa using 15272-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RIBC2 antibody (15272-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RIBC2 Polyclonal antibody

RIBC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15272-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

C22orf11, RIB43A-like with coiled-coils protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse testis tissue, human cervical cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RIBC2 Ready-To-Use IHC Kit. RIBC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15272-1-AP targets RIBC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RIBC2 fusion protein Ag7385 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RIB43A domain with coiled-coils 2
别名 C22orf11, RIB43A-like with coiled-coils protein 2
计算分子量 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC003024
基因名称 RIBC2
Gene ID (NCBI) 26150
RRIDAB_2179714
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H4K1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIBC2 is a microtubule protein and is part of the double microtubules (DMTs) decorated with dynamic proteins in the ciliary axons. It is mainly located in the microtubules of cilia and is involved in flagellated sperm motility(PMID: 40265983).

实验方案

Product Specific Protocols
WB protocol for RIBC2 antibody 15272-1-APDownload protocol
IHC protocol for RIBC2 antibody 15272-1-APDownload protocol
IF protocol for RIBC2 antibody 15272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols