验证数据展示
经过测试的应用
Positive WB detected in | COLO 320 cells, HepG2 cells |
Positive IHC detected in | human colon tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12671-1-AP targets RHPN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | RHPN2 fusion protein Ag3353 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | rhophilin, Rho GTPase binding protein 2 |
别名 | Rhophilin-2, Rhophilin 2, RhoBP, p76RBE, GTP-Rho-binding protein 2 |
计算分子量 | 686 aa, 77 kDa |
观测分子量 | 77 kDa |
GenBank蛋白编号 | BC036447 |
基因名称 | RHPN2 |
Gene ID (NCBI) | 85415 |
RRID | AB_2877872 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8IUC4 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RHPN2 binds specifically to GTP-Rho. It may function in a Rho pathway to limit stress fiber formation and/or increase the turnover of F-actin structures in the absence of high levels of RhoA activity. The MW of this protein is 77 kDa, and this antibody specially recognises the 77 kDa protein.
实验方案
Product Specific Protocols | |
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WB protocol for RHPN2 antibody 12671-1-AP | Download protocol |
IHC protocol for RHPN2 antibody 12671-1-AP | Download protocol |
IF protocol for RHPN2 antibody 12671-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Signal RhoA inhibits neural differentiation in murine stem cells through multiple mechanisms. | ||
Cell Cycle P76RBE silencing inhibits ovarian cancer cell proliferation, migration, and invasion via suppressing the integrin β1/NF-κB pathway.
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J Biochem Mol Toxicol Circ_0000284 upregulates RHPN2 to facilitate pancreatic cancer proliferation, metastasis, and angiogenesis through sponging miR-1179 |