验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HepG2 cells |
| Positive IHC detected in | human colon tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12671-1-AP targets RHPN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3353 Product name: Recombinant human RHPN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 385-686 aa of BC036447 Sequence: LYDHMPEGLTPLATLKNDQQRRQLGKSHLRRAMAHHEESVREASLCKKLRSIEVLQKVLCAAQERSRLTYAQHQEEDDLLNLIDAPSVVAKTEQEVDIILPQFSKLTVTDFFQKLGPLSVFSANKRWTPPRSIRFTAEEGDLGFTLRGNAPVQVHFLDPYCSASVAGAREGDYIVSIQLVDCKWLTLSEVMKLLKSFGEDEIEMKVVSLLDSTSSMHNKSATYSVGMQKTYSMICLAIDDDDKTDKTKKISKKLSFLSWGTNKNRQKSASTLCLPSVGAARPQVKKKLPSPFSLLNSDSSWY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | rhophilin, Rho GTPase binding protein 2 |
| 别名 | Rhophilin-2, Rhophilin 2, RhoBP, p76RBE, GTP-Rho-binding protein 2 |
| 计算分子量 | 686 aa, 77 kDa |
| 观测分子量 | 77 kDa |
| GenBank蛋白编号 | BC036447 |
| 基因名称 | RHPN2 |
| Gene ID (NCBI) | 85415 |
| RRID | AB_2877872 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IUC4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RHPN2 binds specifically to GTP-Rho. It may function in a Rho pathway to limit stress fiber formation and/or increase the turnover of F-actin structures in the absence of high levels of RhoA activity. The MW of this protein is 77 kDa, and this antibody specially recognises the 77 kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RHPN2 antibody 12671-1-AP | Download protocol |
| IHC protocol for RHPN2 antibody 12671-1-AP | Download protocol |
| WB protocol for RHPN2 antibody 12671-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Signal RhoA inhibits neural differentiation in murine stem cells through multiple mechanisms. | ||
Cell Cycle P76RBE silencing inhibits ovarian cancer cell proliferation, migration, and invasion via suppressing the integrin β1/NF-κB pathway.
| ||
J Biochem Mol Toxicol Circ_0000284 upregulates RHPN2 to facilitate pancreatic cancer proliferation, metastasis, and angiogenesis through sponging miR-1179 |
