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RHPN1 Polyclonal antibody

RHPN1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13460-1-AP

Print datasheet

Synonyms

GTP Rho binding protein 1, KIAA1929, ODF5, RHOPHILIN, Rhophilin 1, RHPN, RHPN1

验证数据展示

  • WB analysis using 13460-1-AP

    WB analysis using 13460-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13460-1-AP (RHPN1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 13460-1-AP

    IP experiment of Jurkat using 13460-1-AP

    IP result of anti-RHPN1 (IP:13460-1-AP, 4ug; Detection:13460-1-AP 1:300) with Jurkat cells lysate 3400ug.

经过测试的应用

Positive WB detected inA375 cells, human brain tissue, Jurkat cells, PC-3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13460-1-AP targets RHPN1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen RHPN1 fusion protein Ag3933 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name rhophilin, Rho GTPase binding protein 1
Synonyms GTP Rho binding protein 1, KIAA1929, ODF5, RHOPHILIN, Rhophilin 1, RHPN, RHPN1
Calculated Molecular Weight 670 aa, 74 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC025767
Gene Symbol RHPN1
Gene ID (NCBI) 114822
RRIDAB_2179682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TCX5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RHPN1 antibody 13460-1-APDownload protocol
IP protocol for RHPN1 antibody 13460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols