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验证数据展示

  • WB analysis of rat lung using 26620-1-AP

    WB analysis of rat lung using 26620-1-AP

    rat lung tissue were subjected to SDS PAGE followed by western blot with 26620-1-AP (RHOV Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 26620-1-AP

    IHC staining of human lung cancer using 26620-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26620-1-AP (RHOV Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 26620-1-AP

    IHC staining of human lung cancer using 26620-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26620-1-AP (RHOV Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

RHOV Polyclonal antibody

RHOV Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26620-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

ARHV, CDC42 like GTPase 2, Chp, GTP binding protein like 2, Rho GTPase like protein ARHV, RHOV, WRCH 2, WRCH2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26620-1-AP targets RHOV in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, rat
文献引用反应性human
免疫原 RHOV fusion protein Ag24331 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ras homolog gene family, member V
别名 ARHV, CDC42 like GTPase 2, Chp, GTP binding protein like 2, Rho GTPase like protein ARHV, RHOV, WRCH 2, WRCH2
计算分子量 236 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC112945
基因名称 RHOV
Gene ID (NCBI) 171177
RRIDAB_2880576
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96L33
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RHOV antibody 26620-1-APDownload protocol
IHC protocol for RHOV antibody 26620-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biol Sci

RHOV promotes lung adenocarcinoma cell growth and metastasis through JNK/c-Jun pathway.

Authors - Deyu Zhang
View Article
humanWB,IHC

Front Oncol

Overexpression of RhoV Promotes the Progression and EGFR-TKI Resistance of Lung Adenocarcinoma.

Authors - Hongjin Chen
  • KD Validated
View Article