验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HL-60 cells, mouse testis tissue, mouse heart tissue, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RHOT1/MIRO1 Ready-To-Use IHC Kit. RHOT1/MIRO1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
84055-5-RR targets MIRO1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag15605 Product name: Recombinant human RHOT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 277-618 aa of BC060781 Sequence: DDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKGLFKVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVTVTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHVTQADLKSSTFWLRASFGATVFAVLGFAMYKALLKQR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ras homolog gene family, member T1 |
| 别名 | RHOT1, Rac-GTP-binding protein-like protein, MIRO 1, hMiro 1, EC:3.6.5.- |
| 计算分子量 | 618 aa, 71 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | BC060781 |
| 基因名称 | MIRO1 |
| Gene ID (NCBI) | 55288 |
| RRID | AB_3671623 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q8IXI2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MIRO1 antibody 84055-5-RR | Download protocol |
| IHC protocol for MIRO1 antibody 84055-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |