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验证数据展示

  • WB analysis using 67213-1-Ig

    WB analysis using 67213-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67213-1-Ig (RHOQ/TC10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 67213-1-Ig

    IHC staining of mouse liver using 67213-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67213-1-Ig (RHOQ/TC10 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 67213-1-Ig

    IHC staining of mouse liver using 67213-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67213-1-Ig (RHOQ/TC10 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RHOQ/TC10 Monoclonal antibody

RHOQ/TC10 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67213-1-Ig

产品说明书

CloneNo. 3B9C7

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

ARHQ, Ras like protein TC10, RASL7A, RHOQ, RHOQ/TC10, TC10, TC10A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, rat brain tissue, pig brain tissue, fetal human brain tissue, Jurkat cells, A549 cells
Positive IHC detected inmouse liver tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:8000-1:32000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67213-1-Ig targets RHOQ/TC10 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原

CatNo: Ag12078

Product name: Recombinant human RHOQ protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-205 aa of BC070485

Sequence: MAHGPGALMLKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVSVTVGGKQYLLGLYDTAGQEDYDRLRPLSYPMTDVFLICFSVVNPASFQNVKEEWVPELKEYAPNVPFLLIGTQIDLRDDPKTLARLNDMKEKPICVEQGQKLAKEIGACCYVECSALTQKGLKTVFDEAIIAILTPKKHTVKKRIGSRCINCCLIT

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ras homolog gene family, member Q
别名 ARHQ, Ras like protein TC10, RASL7A, RHOQ, RHOQ/TC10, TC10, TC10A
计算分子量 205 aa, 23 kDa
观测分子量 20-23 kDa
GenBank蛋白编号BC070485
基因名称 RHOQ/TC10
Gene ID (NCBI) 23433
RRIDAB_2882505
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP17081
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for RHOQ/TC10 antibody 67213-1-IgDownload protocol
WB protocol for RHOQ/TC10 antibody 67213-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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