验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, Raji cells, Ramos cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27263-1-AP targets RHOH in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag26127 Product name: Recombinant human RHOH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 116-188 aa of BC014261 Sequence: TQTDQREMGPHRASCVNAMEGKKLAQDVRAKGYLECSALSNRGVQQVFECAVRTAVNQARRRNRRRLFSINEC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ras homolog gene family, member H |
| 别名 | ARHH, TTF, GTP binding protein TTF, GTP-binding protein TTF, Rho-related GTP-binding protein RhoH |
| 计算分子量 | 191 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC014261 |
| 基因名称 | RHOH |
| Gene ID (NCBI) | 399 |
| RRID | AB_3742224 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15669 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RHOH is also named ARHH and TTF. RHOH is a member of the RHO GTPase family, which orchestrates a wide range of cellular processes, such as cytoskeletal reorganization, vesicle trafficking, cell cycle, cell polarity, and cell migration (PMID: 33547421, PMID: 29233866). Unlike typical RHO GTPases that undergo cycling between inactive GDP-bound and active GTP-bound conformation, RHOH lacks intrinsic GTPase activity and remains in a constitutively GTP-bound form (PMID: 11809807). RHOH exhibits high expression in T cells and plays crucial roles in T cell development, activation and differentiation, and undergoes degradation in lysosomes following TCR stimulation (PMID: 17119112, PMID: 30339851, PMID: 19950172). RhoH has been found to regulate proliferation, survival, migration and engraftment of hematopoietic progenitor cells (HPCs), IL-3 signaling in a B cell line, FcεRI-dependent signal transduction in mast cells, and differentiation in eosinophils (PMID: 36108062).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RHOH antibody 27263-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
