验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, human brain tissue, COLO 320 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human colon cancer tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12290-1-AP targets RHOF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2943 Product name: Recombinant human RHOF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-170 aa of BC018208 Sequence: MDAPGALAQTAAPGPGRKELKIVIVGDGGCGKTSLLMVYSQGSFPEHYAPSVFEKYTASVTVGSKEVTLNLYDTAGQEDYDRLRPLSYQNTHLVLICYDVMNPTSYDNVLIKWFPEVTHFCRGIPMVLIGCKTDLRKDKEQLRKLRAAQLEPITYMQVGRGQDPGAQPWL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ras homolog gene family, member F (in filopodia) |
| 别名 | ARHF, Rho family GTPase Rif, Rho in filopodia, RHOF, RIF |
| 计算分子量 | 211 aa, 24 kDa |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | BC018208 |
| 基因名称 | RHOF |
| Gene ID (NCBI) | 54509 |
| RRID | AB_10644126 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HBH0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RHOF antibody 12290-1-AP | Download protocol |
| IHC protocol for RHOF antibody 12290-1-AP | Download protocol |
| IP protocol for RHOF antibody 12290-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem Rho family small GTPase Rif regulates Wnt5a-Ror1-Dvl2 signaling and promotes lung adenocarcinoma progression | ||
Int J Mol Sci An Integrated Multi-Omics Analysis Identifying Immune Subtypes of Pancreatic Cancer | ||
iScience RHOF activation of AKT/β-catenin signaling pathway drives acute myeloid leukemia progression and chemotherapy resistance
| ||
Virchows Arch High expression of RHOF is an effective diagnostic marker and a potential prognostic indicator for primary mediastinal large B-cell lymphoma | ||
EPMA J Deciphering key roles of B cells in prognostication and tailored therapeutic strategies for lung adenocarcinoma: a multi-omics and machine learning approach towards predictive, preventive, and personalized treatment strategies |
