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验证数据展示

  • IF Staining of Human Retina Whole Mount using 11717-1-AP

    IF Staining of Human Retina Whole Mount using 11717-1-AP

    Human Retina Whole Mount generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for RHOD with 11717-1-AP at 1:400, ARR3 1:400 Nuclear stain DAPI (blue). Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

  • IHC staining of human lung cancer using 11717-1-AP

    IHC staining of human lung cancer using 11717-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11717-1-AP (RHOD Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 11717-1-AP

    IHC staining of human lung cancer using 11717-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11717-1-AP (RHOD Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RHOD Polyclonal antibody

RHOD Polyclonal Antibody for IF, IHC, ELISA
Cat No. 11717-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF, IHC, ELISA

别名

ARHD, Rho, Rho related protein HP1, RHOD, RHOHP1, RHOM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman lung cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHuman Retina Whole Mount

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11717-1-AP targets RHOD in IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IF, IHC, ELISA Application Description
经测试反应性 human
免疫原 RHOD fusion protein Ag2291 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ras homolog gene family, member D
别名 ARHD, Rho, Rho related protein HP1, RHOD, RHOHP1, RHOM
计算分子量 210 aa, 23 kDa
GenBank蛋白编号BC001338
基因名称 RHOD
Gene ID (NCBI) 29984
RRIDAB_2877789
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00212
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RhoD is a member of the Rho family of small GTPases, and it has been implicated in integrating actin reorganization and endosome motility. RhoD localizes to early endosomes and recycling endosomes, which indicates its important role in the regulation of endosome trafficking. It is involved in reorganization of the actin cytoskeleton, and it may coordinate membrane transport with the function of the cytoskeleton.

实验方案

Product Specific Protocols
IHC protocol for RHOD antibody 11717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols