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验证数据展示

  • IHC staining of mouse brain using 67502-1-Ig

    IHC staining of mouse brain using 67502-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of K-562 using 67502-1-Ig

    WB analysis of K-562 using 67502-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

RHOBTB3 Monoclonal antibody

RHOBTB3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67502-1-Ig

产品说明书

CloneNo. 1H2E3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

1H2E3, EC:3.6.1.-, KIAA0878, Rho-related BTB domain-containing protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, U2OS cells, HEK-293 cells, HeLa cells, Jurkat cells, pig brain tissue
Positive IHC detected inmouse brain tissue, mouse heart tissue, rat adrenal gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67502-1-Ig targets RHOBTB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag5782

Product name: Recombinant human RHOBTB3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-359 aa of BC041337

Sequence: MSIHIVALGNEGDTFHQDNQPSGLIRTYLGRSPLVSGDESSLLLNAASTVARPVFTEYQASAFGNVKLVVHDCPVWDIFDSDWYTSRNLIGGADIIVIKYNVNDKFSFHEVKDNYIPVIKRALNSVPVIIAAVGTRQNEELPCTCPLCTSDRGSCVSTTEGIQLAKELGATYLELHSLDDFYIGKYFGGVLEYFMIQALNQKTSEKMKKRKMSNSFHGIRPPQLEQPEKMPVLKAEASHYNSDLNNLLFCCQCVDVVFYNPDLKKVVEAHKIVLCAVSHVFMLLFNVKSPTDIQDSSIIRTTQDLFAINRDTAFPGASHESSGNPPLRVIVKDALFCSCLSDILRFIYSGAFQWEELEE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Rho-related BTB domain containing 3
别名 1H2E3, EC:3.6.1.-, KIAA0878, Rho-related BTB domain-containing protein 3
计算分子量 611 aa, 69 kDa
观测分子量 69 kDa
GenBank蛋白编号BC041337
基因名称 RHOBTB3
Gene ID (NCBI) 22836
RRIDAB_2882726
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO94955
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RHOBTB3 antibody 67502-1-IgDownload protocol
IHC protocol for RHOBTB3 antibody 67502-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols