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验证数据展示

  • IHC staining of mouse brain using 67502-1-Ig

    IHC staining of mouse brain using 67502-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of K-562 using 67502-1-Ig

    WB analysis of K-562 using 67502-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

RHOBTB3 Monoclonal antibody

RHOBTB3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67502-1-Ig

产品说明书

CloneNo. 1H2E3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

Rho-related BTB domain-containing protein 3, KIAA0878, EC:3.6.1.-, 1H2E3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, U2OS cells, HEK-293 cells, HeLa cells, Jurkat cells, pig brain tissue
Positive IHC detected inmouse brain tissue, mouse heart tissue, rat adrenal gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67502-1-Ig targets RHOBTB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 RHOBTB3 fusion protein Ag5782 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Rho-related BTB domain containing 3
别名 Rho-related BTB domain-containing protein 3, KIAA0878, EC:3.6.1.-, 1H2E3
计算分子量 611 aa, 69 kDa
观测分子量 69 kDa
GenBank蛋白编号BC041337
基因名称 RHOBTB3
Gene ID (NCBI) 22836
RRIDAB_2882726
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO94955
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RHOBTB3 antibody 67502-1-IgDownload protocol
IHC protocol for RHOBTB3 antibody 67502-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols