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RHOBTB3 Monoclonal antibody

RHOBTB3 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H2E3

Cat No : 67502-1-Ig

Print datasheet

Synonyms

KIAA0878, RHOBTB3

验证数据展示

  • WB analysis of K-562 using 67502-1-Ig

    WB analysis of K-562 using 67502-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67502-1-Ig

    IHC staining of mouse brain using 67502-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 67502-1-Ig

    IF Staining of HeLa using 67502-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, U2OS cells, HEK-293 cells, HeLa cells, Jurkat cells, pig brain tissue
Positive IHC detected inmouse brain tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67502-1-Ig targets RHOBTB3 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Pig, Mouse samples.

Tested Applications IF, IHC, WB, ELISA
Tested Reactivity Human, Pig, Mouse
Immunogen RHOBTB3 fusion protein Ag5782 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Rho-related BTB domain containing 3
Synonyms KIAA0878, RHOBTB3
Calculated Molecular Weight 611 aa, 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC041337
Gene Symbol RHOBTB3
Gene ID (NCBI) 22836
RRIDAB_2882726
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO94955
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RHOBTB3 antibody 67502-1-IgDownload protocol
IHC protocol for RHOBTB3 antibody 67502-1-IgDownload protocol
IF protocol for RHOBTB3 antibody 67502-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols