• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 13945-1-AP

    WB analysis using 13945-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13945-1-AP (RHOBTB3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 13945-1-AP

    IHC staining of mouse heart using 13945-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13945-1-AP (RHOBTB3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 13945-1-AP

    IHC staining of human placenta using 13945-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13945-1-AP (RHOBTB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat adrenal gland using 13945-1-AP

    IHC staining of rat adrenal gland using 13945-1-AP

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 13945-1-AP (RHOBTB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RHOBTB3 Polyclonal antibody

RHOBTB3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13945-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

KIAA0878, RHOBTB3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, K-562 cells
Positive IHC detected inmouse heart tissue, human placenta tissue, rat adrenal gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13945-1-AP targets RHOBTB3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 RHOBTB3 fusion protein Ag5025 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho-related BTB domain containing 3
别名 KIAA0878, RHOBTB3
计算分子量 611 aa, 69 kDa
观测分子量 69 kDa
GenBank蛋白编号BC041337
基因名称 RHOBTB3
Gene ID (NCBI) 22836
RRIDAB_2877992
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94955
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RHOBTB3 antibody 13945-1-APDownload protocol
IHC protocol for RHOBTB3 antibody 13945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

Circular RNA circRHOBTB3 represses metastasis by regulating the HuR-mediated mRNA stability of PTBP1 in colorectal cancer.

Authors - Jiaxin Chen
View Article
humanWB,IP

Cells

RhoBTB3 Functions as a Novel Regulator of Autophagy by Suppressing AMBRA1 Stability

Authors - Kyungho Kim
View Article