验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, human brain tissue, HeLa cells, 3T3-L1 cells, fetal human brain tissue |
| Positive IHC detected in | human brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human pancreas cancer tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15924-1-AP targets RHEB in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag8747 Product name: Recombinant human RHEB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-184 aa of BC016155 Sequence: MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Ras homolog enriched in brain |
| 别名 | RHEB2, Ras homolog enriched in brain, GTP-binding protein Rheb, GTP binding protein Rheb, EC:3.6.5.- |
| 计算分子量 | 184 aa, 20 kDa |
| 观测分子量 | 15-20 kDa |
| GenBank蛋白编号 | BC016155 |
| 基因名称 | RHEB |
| Gene ID (NCBI) | 6009 |
| RRID | AB_2285084 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15382 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RHEB antibody 15924-1-AP | Download protocol |
| IHC protocol for RHEB antibody 15924-1-AP | Download protocol |
| WB protocol for RHEB antibody 15924-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle. | ||
Exp Mol Med A brain somatic RHEB doublet mutation causes focal cortical dysplasia type II. | ||
Metabolism Rheb1 promotes glucose-stimulated insulin secretion in human and mouse β-cells by upregulating GLUT expression.
| ||
Cell Death Dis Synergistic antifibrotic effects of miR-451 with miR-185 partly by co-targeting EphB2 on hepatic stellate cells. | ||
Cell Death Discov MYH9 is crucial for stem cell-like properties in non-small cell lung cancer by activating mTOR signaling. | ||
Acta Pharmacol Sin Intracerebellar upregulation of Rheb(S16H) ameliorates motor dysfunction in mice with SCA2 |
