验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21671-1-AP targets RHBDL3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | RHBDL3 fusion protein Ag16499 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | rhomboid, veinlet-like 3 (Drosophila) |
| 别名 | |
| 计算分子量 | 404 aa, 45 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC100975 |
| 基因名称 | RHBDL3 |
| Gene ID (NCBI) | 162494 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P58872 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RHBDL3 (Rhomboid-related protein 3) is involved in a process known as regulated intramembrane proteolysis (RIP), where proteins are cleaved within the membrane to release functional polypeptides from their membrane anchors. It is expressed in several structures, including the central nervous system, embryo ectoderm, reproductive system, sensory organ, and thymus. RHBDL3 is also a prognostic marker in Glioblastoma multiforme, indicating its potential role in cancer-related pathways. It is expressed in various cell lines, particularly in those related to neuronal function and visual perception, with low cancer specificity.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RHBDL3 antibody 21671-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |