验证数据展示
经过测试的应用
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23181-1-AP targets RHBDF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, pig, monkey |
| 免疫原 |
CatNo: Ag19710 Product name: Recombinant human RHBDF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 81-400 aa of BC016034 Sequence: KNPAYLKSVSLQEPRSRWQESSEKRPGFRRQASLSQSIRKGAAQWFGVSGDWEGQRQQWQRRSLHHCSMRYGRLKASCQRDLELPSQEAPSFQGTESPKPCKMPKIVDPLARGRAFRHPEEMDRPHALHPPLTPGVLSLTSFTSVRSGYSHLPRRKRMSVAHMSLQAAAALLKGRSVLDATGQRCRVVKRSFAFPSFLEEDVVDGADTFDSSFFSKEEMSSMPDDVFESPPLSASYFRGIPHSASPVSPDGVQIPLKEYGRAPVPGPRRGKRIASKVKHFAFDRKKRHYGLGVVGNWLNRSYRRSISSTVQRQLESFDSH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | rhomboid 5 homolog 2 (Drosophila) |
| 别名 | Rhomboid family member 2, Rhomboid 5 homolog 2, RHBDL6, RHBDL5, iRhom2 |
| 计算分子量 | 856 aa, 97 kDa |
| GenBank蛋白编号 | BC016034 |
| 基因名称 | RHBDF2 |
| Gene ID (NCBI) | 79651 |
| RRID | AB_2879224 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6PJF5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The gene for rhomboid 5 homolog 2 (RHBDF2) is a protein-coding gene that encodes rhombus protease, iRhom2, which participates in important biological processes. RHBDF2 is significantly upregulated in tumors and has pro-tumor effects.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RHBDF2 antibody 23181-1-AP | Download protocol |
| IHC protocol for RHBDF2 antibody 23181-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Int RHBDF2 gene functions are correlated to facilitated renal clear cell carcinoma progression.
| ||
FEBS Open Bio RHBDF2 is correlated with immune infiltrates in hepatocellular carcinoma and may have potential as a biomarker | ||
Biotechnol Genet Eng Rev The prognostic value of RHBDF2 in Pan-Cancer, and its correlation with cell Adhesion of Hepatocellular Carcinoma | ||
J Virol The Papain-Like Protease of Porcine Reproductive and Respiratory Syndrome Virus Impedes STING Translocation from the Endoplasmic Reticulum to the Golgi Apparatus by Deubiquitinating STIM1 | ||
Vet Microbiol Porcine reproductive and respiratory syndrome virus infection activates ADAM17 to induce inflammatory responses |
