验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, HCT 116 cells, HEK-293 cells, mouse testis tissue, PC-3 cells, human testis tissue, mouse colon tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
产品信息
20869-1-AP targets RHBDD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag14932 Product name: Recombinant human RHBDD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 13-106 aa of BC027900 Sequence: SSSVGYPGRQYYFNSSGSSGYQDYYPHGRPDHYEEAPRNYDTYTAGLSEEEQLERALQASLWDRGNTRNSPPPYGFHLSPEEMRRQRLHRFDSQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | rhomboid domain containing 1 |
| 别名 | EC:3.4.21.105, HSD 50, HSD-50, RHBDL4, rhomboid domain containing 1 |
| 计算分子量 | 315 aa, 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC027900 |
| 基因名称 | RHBDD1 |
| Gene ID (NCBI) | 84236 |
| RRID | AB_10733756 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TEB9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RHBDD1 (Rhomboid Domain Containing 1), also known as RHBDL4), is a rhomboid endomembrane serine protease located on the endoplasmic reticulum membrane. It plays a key role in cellular protein quality control, apoptosis and lipid homeostasis. RHBDD1 is capable of cleaving misfolded transmembrane proteins on the endoplasmic reticulum membrane, thereby facilitating their transport to the cytoplasm for degradation by the proteasome.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RHBDD1 antibody 20869-1-AP | Download protocol |
| WB protocol for RHBDD1 antibody 20869-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem AAA-ATPase valosin-containing protein (VCP) binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4. | ||
Environ Toxicol Suppression of rhomboid domain-containing 1 produces anticancer effects in pancreatic adenocarcinoma through affection of the AKT/GSK-3β/β-catenin pathway.
| ||
Cell Death Dis Long noncoding RNA AFAP1-AS1 promotes tumor progression and invasion by regulating the miR-2110/Sp1 axis in triple-negative breast cancer. | ||
Genes Cells Mouse transient receptor potential melastatin 2 (TRPM2) isoform 7 attenuates full-length mouse TRPM2 activity through reductions in its expression by targeting it to ER-associated degradation
| ||
