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验证数据展示

  • WB analysis of mouse brain using 25836-1-AP

    WB analysis of mouse brain using 25836-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25836-1-AP (RGSL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 25836-1-AP

    IHC staining of human testis using 25836-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25836-1-AP (RGSL1 Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 25836-1-AP

    IHC staining of human testis using 25836-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25836-1-AP (RGSL1 Antibody) at dilution of 1:100 (under 40x lens).

RGSL1 Polyclonal antibody

RGSL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25836-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

RGSL, RGSL1, RGSL2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25836-1-AP targets RGSL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 RGSL1 fusion protein Ag20269 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulator of G-protein signaling like 1
别名 RGSL, RGSL1, RGSL2
计算分子量 1076 aa, 126 kDa
观测分子量 125 kDa
GenBank蛋白编号BC142944
基因名称 RGSL1
Gene ID (NCBI) 353299
RRIDAB_2880263
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA5PLK6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RGSL1, also named as RGSL and RGSL2, belongs to the RSG family. RGSL1 is involved in the G-protein signaling regulation with a suggested role in prostate carcinogenesis (PMID: 28662289). RGSL1 has 6 isoforms with the molecular mass of 47-63, 71 and 126 kDa. RGSL1 may interact with other markers in the development of breast cancer (PMID: 21135262).

实验方案

Product Specific Protocols
WB protocol for RGSL1 antibody 25836-1-APDownload protocol
IHC protocol for RGSL1 antibody 25836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols