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验证数据展示

  • WB analysis using 88404-1-RR (same clone as 88404-1-PBS)

    WB analysis using 88404-1-RR (same clone as 88404-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88404-1-RR (RGS5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88404-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 88404-1-RR (same clone as 88404-1-PBS)

    IF Staining of NIH/3T3 using 88404-1-RR (same clone as 88404-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RGS5 antibody (88404-1-RR, Clone: 260952D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 88404-1-PBS in a different storage buffer formulation.

RGS5 Recombinant monoclonal antibody, PBS Only

RGS5 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 88404-1-PBS

产品说明书

CloneNo. 260952D7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

Regulator of G-protein signaling 5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

88404-1-PBS targets RGS5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2196

Product name: Recombinant human RGS5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-181 aa of BC030059

Sequence: MCKGLAALPHSCLERAKEIKIKLGILLQKPDSVGDLVIPYNEKPEKPAKTQKTSLDEALQWRDSLDKLLQNNYGLASFKSFLKSEFSEENLEFWIACEDYKKIKSPAKMAEKAKQIYEEFIQTEAPKEVNIDHFTKDITMKNLVEPSLSSFDMAQKRIHALMEKDSLPRFVRSEFYQELIK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 regulator of G-protein signaling 5
别名 Regulator of G-protein signaling 5
计算分子量 181 aa, 21 kDa
观测分子量 42 kDa
GenBank蛋白编号BC030059
基因名称 RGS5
Gene ID (NCBI) 8490
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15539
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RGS5 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. It binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. The 42kDa band recognized by this antibody is a RGS5-RGS5 dimer. (PMID:17762159)

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