验证数据展示
经过测试的应用
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28231-1-AP targets RGS22 in IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag28156 Product name: Recombinant human RGS22 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 250-499 aa of BC092411 Sequence: HPRTKKDPSKTNKLISEFEEEEGEEEEVSVSLQDTPSQALLRVYLEKKQDVDESLTMHFSTCEEFLSSYIYFILRGAIQQIVGKPVGETPDYINFNNITKVSFDDCFESIHGKNFLSELVQTTKERSEEIEQTSLSSKNESAGPESRADWCISHRTYDIGNRKEFERFKKFIKGTLGERYWWLWMDIERLKVLKDPGRHQRHLEKMKKCYLVSNGDYYLSAEILSKFKLLDGSQWNEEHLRNIQSEVLKP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | regulator of G-protein signaling 22 |
| 别名 | regulator of G protein signaling 22, Regulator of G-protein signaling 22, RGS 22 |
| 计算分子量 | 1263 aa, 147 kDa |
| GenBank蛋白编号 | BC092411 |
| 基因名称 | RGS22 |
| Gene ID (NCBI) | 26166 |
| RRID | AB_3742287 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NE09 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The RGS22 protein (Regulator of G-protein signaling 22) belongs to the regulators of G-protein signaling (RGS) protein family and is a member of the RA/RGS subfamily. Its protein structure contains at least three conserved RGS domains. RGS22 primarily functions as a GTPase-activating protein (GAP) by binding to the G protein α subunit and enhancing its GTPase activity, thereby promoting the conversion of G proteins from an active state to an inactive state, which negatively regulates downstream signal transduction of G protein-coupled receptors.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RGS22 antibody 28231-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
