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RGS17 Polyclonal antibody

RGS17 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 12549-1-AP

Print datasheet

Synonyms

hRGS17, RGS 17, RGS17, RGSZ2

验证数据展示

  • WB analysis of mouse lung using 12549-1-AP

    WB analysis of mouse lung using 12549-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12549-1-AP (RGS17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 12549-1-AP

    IHC staining of human testis using 12549-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 12549-1-AP (RGS17 Antibody) at dilution of 1:50.

  • IHC staining of human testis using 12549-1-AP

    IHC staining of human testis using 12549-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 12549-1-AP (RGS17 Antibody) at dilution of 1:50.

  • IF Staining of MCF-7 using 12549-1-AP

    IF Staining of MCF-7 using 12549-1-AP

    Immunofluorescent analysis of MCF-7 cells using 12549-1-AP (RGS17 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse lung tissue, MCF-7 cells, PC-3 cells, SH-SY5Y cells
Positive IHC detected inhuman testis tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12549-1-AP targets RGS17 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Immunogen RGS17 fusion protein Ag3399 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name regulator of G-protein signaling 17
Synonyms hRGS17, RGS 17, RGS17, RGSZ2
Calculated Molecular Weight 210 aa, 24 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC013117
Gene Symbol RGS17
Gene ID (NCBI) 26575
RRIDAB_2179954
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGC6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RGS17 antibody 12549-1-APDownload protocol
IHC protocol for RGS17 antibody 12549-1-APDownload protocol
IF protocol for RGS17 antibody 12549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Signal

Altered expression and function of regulator of G-protein signaling-17 (RGS17) in hepatocellular carcinoma.

Authors - Eugene Sokolov
View Article
ratWB,IF

Sci Rep

Regulator of G protein signaling 17 represents a novel target for treating cisplatin induced hearing loss.

Authors - Asmita Dhukhwa
View Article
humanWB, IHC

J Cancer

Deregulation of RGS17 Expression Promotes Breast Cancer Progression.

Authors - Yuhua Li
View Article