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验证数据展示

  • WB analysis of mouse thymus using 11163-1-AP

    WB analysis of mouse thymus using 11163-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11163-1-AP (RGS10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 11163-1-AP

    IHC staining of human stomach using 11163-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11163-1-AP (RGS10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

RGS10 Polyclonal antibody

RGS10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11163-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Regulator of G-protein signaling 10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse thymus tissue, K-562 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11163-1-AP targets RGS10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原

CatNo: Ag1644

Product name: Recombinant human RGS10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-181 aa of BC009361

Sequence: MFNRAVSRLSRKRPPSDIHDSDGSSSSSHQSLKSTAKWAASLENLLEDPEGVKRFREFLKKEFSEENVLFWLACEDFKKMQDKTQMQEKAKEIYMTFLSSKASSQVNVEGQSRLNEKILEEPHPLMFQKLQDQIFNLMKYDSYSRFLKSDLFLKHKRTEEEEEDLPDAQTAAKRASRIYNT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulator of G-protein signaling 10
别名 Regulator of G-protein signaling 10
计算分子量 21 kDa
观测分子量 20-22 kDa
GenBank蛋白编号BC009361
基因名称 RGS10
Gene ID (NCBI) 6001
RRIDAB_10596933
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43665
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RGS10 antibody 11163-1-APDownload protocol
IHC protocol for RGS10 antibody 11163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int Endod J

RGS10 negatively regulates apical periodontitis via TFEB-mediated autophagy in BABL/c mice model and in vitro

Authors - Jiaxin Li
View Article
mouseWB

Oral Dis

Inhibition of Rgs10 aggravates periodontitis with collagen-induced arthritis via the NF-κB pathway.

Authors - Wei-Cheng Chan
  • KD Validated
View Article
mouseWB

J Endod

Inhibition of RGS10 Aggravates Periapical Periodontitis via Upregulation of the NF-κB Pathway

Authors - Wei Wei
View Article