验证数据展示
经过测试的应用
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31636-1-AP targets RGPD5 in IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag36253 Product name: Recombinant human RGPD5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-230 aa of NM_001037866 Sequence: MRRSKADVERYVASVLGLTPSPRQKSMKGFYFAKLYYEAKEYDLAKKYICTYINVQERDPKAHRFLGLLYELEENTEKAVECYRRSVELNPTQKDLVLKIAELLCKNDVTDGRAKYWVERAAKLFPGSPAIYKLKEQLLDCEGEDGWNKLFDLIQSELYVRPDDVHVNIRLVELYRSTKRLKDAVAHCHEAERNIALRSSLEWNSCVVQTLKEYLESLQCLESDKSDWQA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RANBP2-like and GRIP domain containing 5 |
| 别名 | BS 63, HEL161, RANBP2 like and GRIP domain containing 5, RanBP2L1, RanBP2L2 |
| 计算分子量 | 198kDa |
| GenBank蛋白编号 | NM_001037866 |
| 基因名称 | RGPD5 |
| Gene ID (NCBI) | 84220 |
| RRID | AB_3742459 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q99666 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The RGPD5 protein, full name RANBP2-like and GRIP domain containing 5, belongs to the RANBP2 (Ran-binding protein 2) gene family and is a member of this family generated through gene duplication. This protein is a large macromolecule whose functions are involved in the regulation of intracellular nucleocytoplasmic transport. It contains multiple functional domains, including the RanBD domain capable of binding to the small GTPase RAN, the GRIP domain that mediates protein-protein interactions, and regions associated with SUMO E3 ligase activity.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RGPD5 antibody 31636-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |




