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验证数据展示

  • WB analysis using 26626-1-AP

    WB analysis using 26626-1-AP

    HepG2 cells, RT-4 cells, Caco-2 cells lysates were subjected to SDS PAGE followed by western blot with 26626-1-AP (RGL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RGL3 Polyclonal antibody

RGL3 Polyclonal Antibody for WB, ELISA
Cat No. 26626-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

RalGDS like 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRT-4 cells, HepG2 cells, Caco-2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26626-1-AP targets RGL3 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 RGL3 fusion protein Ag24329 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ral guanine nucleotide dissociation stimulator-like 3
别名 RalGDS like 3
计算分子量 710 aa, 78 kDa
观测分子量78 kDa
GenBank蛋白编号BC101756
基因名称 RGL3
Gene ID (NCBI) 57139
RRIDAB_3669556
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3MIN7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RGL3 is a guanine nucleotide exchange factor. RGL3 mRNA is commonly detectable in several visceral organs (e.g. kidney, heart, liver, and lung) in the mouse and human. The RGL3 protein mainly localizes in the cytoplasm when expressed in fibroblasts. Yeast two-hybrid assay indicated that RGL3 could interact with Rap1, Rap2, H-Ras, N-Ras, and R-Ras but failed to interact efficiently with Ral and Rho (PMID: 17382517).

实验方案

Product Specific Protocols
WB protocol for RGL3 antibody 26626-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols