验证数据展示
经过测试的应用
| Positive WB detected in | RT-4 cells, HepG2 cells, Caco-2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26626-1-AP targets RGL3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | RGL3 fusion protein Ag24329 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ral guanine nucleotide dissociation stimulator-like 3 |
| 别名 | RalGDS like 3 |
| 计算分子量 | 710 aa, 78 kDa |
| 观测分子量 | 78 kDa |
| GenBank蛋白编号 | BC101756 |
| 基因名称 | RGL3 |
| Gene ID (NCBI) | 57139 |
| RRID | AB_3669556 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q3MIN7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RGL3 is a guanine nucleotide exchange factor. RGL3 mRNA is commonly detectable in several visceral organs (e.g. kidney, heart, liver, and lung) in the mouse and human. The RGL3 protein mainly localizes in the cytoplasm when expressed in fibroblasts. Yeast two-hybrid assay indicated that RGL3 could interact with Rap1, Rap2, H-Ras, N-Ras, and R-Ras but failed to interact efficiently with Ral and Rho (PMID: 17382517).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RGL3 antibody 26626-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |