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验证数据展示

  • WB analysis using 20188-1-AP

    WB analysis using 20188-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20188-1-AP (RFXAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 20188-1-AP

    IF Staining of HeLa using 20188-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 20188-1-AP (RFXAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

RFXAP Polyclonal antibody

RFXAP Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20188-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Regulatory factor X-associated protein, RFX associated protein, RFX DNA-binding complex 36 kDa subunit, RFX-associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, MOLT-4 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20188-1-AP targets RFXAP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14100

Product name: Recombinant human RFXAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 80-272 aa of BC026088

Sequence: DGEEEAGEDEADLLDTSDPPGGGESAASLEDLEDEETHSGGEGSSGGARRRGSGGGSMSKTCTYEGCSETTSQVAKQRKPWMCKKHRNKMYKDKYKKKKSDQALNCGGTASTGSAGNVKLEESADNILSIVKQRTGSFGDRPARPTLLEQVLNQKRLSLLRSPEVVQFLQKQQQLLNQQVLEQRQQQFPGTSM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulatory factor X-associated protein
别名 Regulatory factor X-associated protein, RFX associated protein, RFX DNA-binding complex 36 kDa subunit, RFX-associated protein
计算分子量 272 aa, 28 kDa
观测分子量 36 kDa
GenBank蛋白编号BC026088
基因名称 RFXAP
Gene ID (NCBI) 5994
RRIDAB_10697835
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00287
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RFXAP is a subunit of the RFX complex that binds to the X-box of MHC II promoters. It's involved in Bare lymphocyte syndrome 2 (BLS2).

实验方案

Product Specific Protocols
WB protocol for RFXAP antibody 20188-1-APDownload protocol
IF protocol for RFXAP antibody 20188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols