• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HeLa using 20188-1-AP

    WB analysis of HeLa using 20188-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20188-1-AP (RFXAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 20188-1-AP

    IF Staining of HeLa using 20188-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 20188-1-AP (RFXAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

RFXAP Polyclonal antibody

RFXAP Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20188-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

RFX associated protein, RFXAP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20188-1-AP targets RFXAP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 RFXAP fusion protein Ag14100 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulatory factor X-associated protein
别名 RFX associated protein, RFXAP
计算分子量 272 aa, 28 kDa
观测分子量 23-26 kDa
GenBank蛋白编号BC026088
基因名称 RFXAP
Gene ID (NCBI) 5994
RRIDAB_10697835
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00287
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RFXAP is a subunit of the RFX complex that binds to the X-box of MHC II promoters. It's involved in Bare lymphocyte syndrome 2 (BLS2).

实验方案

Product Specific Protocols
WB protocol for RFXAP antibody 20188-1-APDownload protocol
IF protocol for RFXAP antibody 20188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols